tritc conjugated concanavalin a  (Vector Laboratories)

Journal: bioRxiv

Article Title: C. albicans ergosterol modulates the antifungal response of human neutrophils by masking β-glucan

doi: 10.64898/2026.05.18.721578

Figure Lengend Snippet: HPIC analysis of cell wall (A) glucosamine, (B) glucose and (C) mannose composition in WT and ERG4 -depleted strain. (D) Representative confocal micrographs of mid-log phase cultures of WT and ERG4 -depleted strains stained with a human Fc-Dectin-1 fusion protein ((β-1,3-glucan), calcofluor white (CFW; chitin), and Alexa Fluor 488 conjugated concanavalin A (ConA; mannan). Scale bar, 15 μm. (E-G) Quantification of the relative mean fluorescence intensity in WT and ERG4 -depleted strains from (D) . Each dot represents a field of view (n=3 biological replicates). (H-J) Quantification of the mean fluorescence intensity (MFI) levels of exposed β-1,3-glucan, total chitin and mannan by flow cytometry. MFI was normalised to stained WT values of each experimental repeat (n=3). Error bars represent mean ± SEM. Significance was calculated using a two-tailed unpaired Student’s t-test. ** P < 0.01, **** P < 0.0001 ns: P > 0.05.

Article Snippet: Fixed cells were washed with PBS and stained in 96-well plates using the following probes: (1) β-1,3-glucan was labelled with 5 μg/mL Dectin-1-Fc (R&D Systems), followed by goat anti-human IgG conjugated to Alexa Fluor 488 (Thermo Fisher Scientific), (2) chitin was stained with 10 μg/mL Calcofluor White (Sigma), (3) mannan was detected with 50 μg/mL TRITC-conjugated concanavalin A (Thermo Fisher Scientific).

Techniques: Staining, Fluorescence, Flow Cytometry, Two Tailed Test

Journal: iScience

Article Title: BRCA1 mediates protein homeostasis through the ubiquitination of PERK and IRE1

doi: 10.1016/j.isci.2022.105626

Figure Lengend Snippet:

Article Snippet: Concanavalin-A TRITC conjugated , Thermo Fisher , Cat# C860.

Techniques: Virus, Recombinant, Ubiquitin Proteomics, Extraction, SYBR Green Assay, Isolation, Control, Plasmid Preparation, Software

Journal: IOP Conference Series: Materials Science and Engineering

Article Title: Design of nanostructured coating to prevent biofilm formation on surfaces

doi: 10.1088/1757-899x/1265/1/012003

Figure Lengend Snippet: Figure 4. Biofilm growth after 72h in stagnant fluid. Cells were stained with the green fluorescent dye SYTO® 9, while the red fluorescent dye Tetra-methyl-rhodamine (TRITC) conjugated Concanavalin A was used to stain α-mannose residues within the biofilm matrix. Different concentration of non- linked Mag-2 are used: a) Control sample, b) 25µM, c) 50 µM. Image dimension 230x230 µm This preliminary investigation of the efficiency of Mag-2 shows it is possible to develop a further implementation of the same technology with the goal to realize a nanostructured coating agent on solid surfaces for antibiofilm purpose. The same methodic used to evaluate biofilm morphology under AMP influence in stagnant fluid can be applied for both shaken vessel and in flow setup methods. Using as control both data represented in Fig.3 and Fig. 4 a comparison between biofilm growth under immobilized-AMPs, non-immobilized AMPs, and absence of AMPs can be made.

Article Snippet: Following the manufacture protocols (ThermoFisher, 2018), cells were stained using the green, fluorescent dye SYTO® 9 (3 μL/mL), while EPS lectins were stained with the red dye Texas Red TRITC conjugate CoA (100 μg/mL) (Tetra-methyl-rhodamine conjugated Concanavalin A, Molecular Probes, Invitrogen, Carlsbad, CA, USA).

Techniques: Staining, Concentration Assay, Control, Comparison

Journal: Glia

Article Title: Deletion of Tgfβ signal in activated microglia prolongs hypoxia‐induced retinal neovascularization enhancing Igf1 expression and retinal leukostasis

doi: 10.1002/glia.24218

Figure Lengend Snippet: Tgfβ signal deletion in microglia did not affect structure of vessels and vascular development but, promoted the adhesion of leukocytes via enhancing chemoattractant. (a) The schematic shows the protocol that was used to generate data for this figure. (b) GS‐lectin labeled retinal vessels at superficial, intermediate, and deep plexus 8 weeks after tamoxifen injection from P9 to P14. Scale bars = 100 μm. (f) TRITC‐conjugated Concanavalin a (con a) lectin labeled leukocytes adhering to retinal capillary endothelium. Scale bars = 500 μm. (b) the number of branching points per 400 μm 2 was evaluated (control n = 6, Tgfβbr2 KO (ΔMG) n = 7). (c) NG2 positive pericytes at superficial, intermediate, and deep plexus 8 weeks after daily tamoxifen injections on P9 to P14 (scale bars = 100 μm). (e) the number of NG2 positive pericytes per 400 μm 2 was evaluated (control n = 6, Tgfβbr2 KO (ΔMG) n = 5). (f) TRITC‐conjugated Concanavalin a (con a) lectin labeled leukocytes adhering to retinal capillary endothelium (scale bars = 500 μm). (g) Adherent leukocytes to retinal capillaries are visualized by TRITC‐conjugated con a lectin at each layer. There are more leukocytes in all layers in Tgfβbr2 KO (ΔMG) mice compared to control (scale bars = 100 μm). (h) the number of adherent leukocytes labeled with con a lectin in Tgfβbr2 KO (ΔMG) mice was higher than those observed in control mice ( n = 4 each). Data are mean ± SEM. p Values were calculated using a two‐tailed Student's t ‐test, * p < .05.

Article Snippet: The retinal vasculature and adherent leukocytes were imaged by perfusion labeling with TRITC‐conjugated Concanavalin A (Con A) lectin (Vector Laboratories), as described previously (Joussen et al., ; Okunuki et al., ).

Techniques: Labeling, Injection, Two Tailed Test